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| Plasmid DNAs and single-stranded DNAs We recommend that plasmid templates be purified using Montage® Plasmid Miniprep Kits, BioRad's Quantum® miniprep kits, Promega'sWizard® miniprep, or Qiagen Qiaprep® miniprep kits. We ask (but do not require) that you send a gel photograph along with your samples so we can verify sample quality before performing the sequencing reactions. We can process samples submitted in all common microtube sizes, although we prefer that samples be submitted in 1.5 ml tubes. If you have a large number of samples, they can also be sent in 96-well microtiter plates designed for PCR. (Please don't send samples in regular microtiter plates (i.e.,those designed for immunoassays). Those plates are brittle and sometimes crack during shipment, resulting in sample loss.) Samples can be submitted in two ways: 1. Sample DNA and primer premixed in one tube total volume 10 ul. Primer final concentration should be 2.5 uM (2.5 picomoles/uL. 2. 10 ul of DNA sample in one tube and 10 ul of primer in a separate tube. The concentration of the primer sent separately should be 5 uM (5 picomoles/ul). Be sure to label each tube (preferably on both sides) with the sequence name listed on the submission sheet. For double-stranded DNA less than 6 Kb, 1 ug of DNA should be sent; for Double-stranded DNA larger than 6 Kb, 1.5 ug should be sent. A total of 0.5 ug of DNA should be sent if you are providing single-stranded DNA. The sample must be in water or Tris buffer (not TE)! |
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